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Histopathological and immunohistochemical findings. ( A ) Hematoxylin and eosin–stained sections examined showed a monomorphic population of small round cells arranged in trabeculae and lobules with intervening connective tissue septae (400x). ( B ) The tumor cells were seen invading the bony fragments (400x). ( C–F ) Immunohistochemistry exhibited positive immunostaining for CD99 ( C ) and FLI-1 ( D ) with negative staining for glial <t>fibrillary</t> acidic protein <t>(GFAP)</t> ( E ). A K i -67 proliferation index of 50% was seen (400x).
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Histopathological and immunohistochemical findings. ( A ) Hematoxylin and eosin–stained sections examined showed a monomorphic population of small round cells arranged in trabeculae and lobules with intervening connective tissue septae (400x). ( B ) The tumor cells were seen invading the bony fragments (400x). ( C–F ) Immunohistochemistry exhibited positive immunostaining for CD99 ( C ) and FLI-1 ( D ) with negative staining for glial fibrillary acidic protein (GFAP) ( E ). A K i -67 proliferation index of 50% was seen (400x).

Journal: Journal of Neurological Surgery Reports

Article Title: Ewing's Sarcoma of the Frontal Bone with Intracranial Extension

doi: 10.1055/a-2649-0851

Figure Lengend Snippet: Histopathological and immunohistochemical findings. ( A ) Hematoxylin and eosin–stained sections examined showed a monomorphic population of small round cells arranged in trabeculae and lobules with intervening connective tissue septae (400x). ( B ) The tumor cells were seen invading the bony fragments (400x). ( C–F ) Immunohistochemistry exhibited positive immunostaining for CD99 ( C ) and FLI-1 ( D ) with negative staining for glial fibrillary acidic protein (GFAP) ( E ). A K i -67 proliferation index of 50% was seen (400x).

Article Snippet: Immunohistochemistry was performed and tumor cells exhibited positive expression for CD99 (rabbit monoclonal, clone EP8, Biocare Medical), vimentin (mouse monoclonal, clone V9, Biocare Medical), and FLI-1 (mouse monoclonal, clone G146–222, BioSB) while being negative for glial fibrillary acidic protein (GFAP) (mouse monoclonal, clone GA-5, Biocare Medical) and synaptophysin (mouse monoclonal, clone 27G12, Biocare Medical).

Techniques: Immunohistochemical staining, Staining, Immunohistochemistry, Immunostaining, Negative Staining